Development of a monoclonal antibody for the detection of anti-canine CD20 chimeric antigen receptor expression on canine CD20 chimeric antigen receptor-transduced T cells.

Osamu Sakai
Shoji Ogino
Toshihiro Tsukui
Masaya Igase
Takuya Mizuno

Chimeric antigen receptor (CAR) CAR-T cell therapy targeting CD20 can be a novel adoptive cell therapy for canine patients with B-cell malignancy. After injection of the CAR-T cells in vivo, monitoring circulating CAR-T cells is essential to prove in vivo persistence of CAR-T cells. In this study, we developed a novel monoclonal antibody against canine CD20 CAR, whose single-chain variable fragment was derived from the our previously reported anti-canine CD20 therapeutic antibody. Furthermore, we proved that this monoclonal antibody can detect therapeutic anti-canine CD20 chimeric antibody in the serum from healthy beagle dogs injected with the therapeutic antibody for safety study. This monoclonal antibody is a useful tool for monitoring both canine CD20-CAR-T cells and anti-canine CD20 therapeutic antibody for canine lymphoma.

The Journal of veterinary medical science

83

10

1495

1499

10.1292/jvms.21-0326

https://doi.org/10.1292/jvms.21-0326
https://www.ncbi.nlm.nih.gov/pubmed/34408098
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569873