VOXEL-BASED MORPHOMETRY OF THE MARMOSET BRAIN: IN VIVO DETECTION OF VOLUME LOSS IN THE SUBSTANTIA NIGRA OF THE MPTP-TREATED PARKINSON'S DISEASE MODEL

K. Hikishima
K. Ando
Y. Komaki
K. Kawai
R. Yano
T. Inoue
T. Itoh
M. Yamada
S. Momoshima
H. J. Okano
H. Okano

Movement dysfunction in Parkinson's disease (PD) is caused by the degeneration of dopaminergic (DA) neurons in the substantia nigra (SN). Here, we established a method for voxel-based morphometry (VBM) and automatic tissue segmentation of the marmoset monkey brain using a 7-T animal scanner and applied the method to assess DA degeneration in a PD model, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated animals, with tyrosine-hydroxylase staining. The most significant decreases of local tissue volume were detected in the bilateral SN of MPTP-treated marmoset brains (-53.0% in right and -46.5% in left) and corresponded with the location of DA neurodegeneration found in histology (-65.4% in right). In addition to the SN, the decreases were also confirmed in the locus coeruleus, and lateral hypothalamus. VBM using 7-T MRI was effective in detecting volume loss in the SN of the PD-model marmoset. This study provides a potential basis for the application of VBM with ultra-high field MRI in the clinical diagnosis of PD. The developed method may also offer value in automatic whole-brain evaluation of structural changes for the marmoset monkey. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

NEUROSCIENCE

PERGAMON-ELSEVIER SCIENCE LTD

300

585

592

10.1016/j.neuroscience.2015.05.041

https://doi.org/10.1016/j.neuroscience.2015.05.041
https://www.ncbi.nlm.nih.gov/pubmed/26012491
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