Characterization of the glycoproteins of bat-derived influenza viruses

Junki Maruyama
Naganori Nao
Hiroko Miyamoto
Ken Maeda
Hirohito Ogawa
Reiko Yoshida
Manabu Igarashi
Ayato Takada

Recently found bat-derived influenza viruses (BatlVs) have hemagglutinin (HA) and neuraminidase (NA) gene segments distinct from those of previously known influenza A viruses. However, pathogenicities of these BatlVs remain unknown since infectious virus strains have not been isolated yet. To gain insight into the biological properties of BatlVs, we generated vesicular stomatitis viruses (VSVs) pseudotyped with the BatIV HA and NA. We found that VSVs pseudotyped with BatIV HAs and NAs efficiently infected particular bat cell lines but not those derived from primates, and that proteolytic cleavage with a trypsinlike protease was necessary for HA-mediated virus entry. Treatment of the susceptible bat cells with some enzymes and inhibitors revealed that BatIV HAs might recognize some cellular glycoproteins as receptors rather than the sialic acids used for the other known influenza viruses. These data provide fundamental information on the mechanisms underlying the cellular entry and host restriction of BatIVs. (C) 2015 Elsevier Inc. All rights reserved.

VIROLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

488

43

50

10.1016/j.virol.2015.11.002

https://doi.org/10.1016/j.virol.2015.11.002
https://www.ncbi.nlm.nih.gov/pubmed/26605499
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