Utilization of meat juice from market-purchased pork for serological surveillance of mosquito-borne arboviruses

Kuwata R, Thu LNA, Phuong DTM, Takeishi M, Inoue Y, Ohta N, Flores Lino VI, Ogawa H, Watanabe S, Shimoda H, Suzuki Y, Hang PT, Amano M, Abe H, Higa Y, Isawa H, Maeda K, Phung LD, Hasebe F, Kasai S.

This study evaluated the utility of meat juice (MJ) from market-purchased pork in Japan and Vietnam for serological surveillance of mosquito-borne viruses, specifically Japanese encephalitis virus (JEV) and Getah virus (GETV). We confirmed the presence of specific antibodies against JEV and GETV in the MJ samples using enzyme-linked immunosorbent assay and virus neutralization test. The lack of correlation between JEV- and GETV-specific optical density values suggests independent infections in Vietnamese pig populations. The high seroprevalence observed in Vietnam highlights the practical utility of MJ as a viable alternative to serum for effective large-scale arboviral surveillance in field settings. These findings underscore the need for comprehensive mosquito-borne virus control in swine health management.

J Vet Med Sci