Survival rate and expression of Heat-shock protein 70 and Frost genes after temperature stress in Drosophila melanogaster lines that are selected for recovery time from temperature coma

Hiroko Udaka
Chiaki Ueda
Shin G. Goto

In this study we investigated the physiological mechanisms underlying temperature tolerance using Drosophila melanogaster lines with rapid intermediate or slow recovery from heat or chill coma that were established by artificial selection or by free recombination without selection Specifically we focused on the relationships among their recovery from heat or chill coma survival after severe heat or cold and survival enhanced by rapid cold hardening (RCH) or heat hardening The recovery time from heat coma was not related to the survival rate after severe heat The line with rapid recovery from chill coma showed a higher survival rate after severe cold exposure and therefore the same mechanisms are likely to underlie these phenotypes The recovery time from chill coma and survival rate after severe cold were unrelated to RCH-enhanced survival We also examined the expression of two genes Heat-shock protein 70 (Hsp70)and Frost in these lines to understand the contribution of these stress-inducible genes to intraspecific variation in recovery from temperature coma The line showing rapid recovery from heat coma did not exhibit higher expression of Hsp70 and Frost In addition Hsp70 and Frost transcription levels were not correlated with the recovery time from chill coma Thus Hsp70 and Frost transcriptional regulation was not involved in the intraspecific variation in recovery from temperature coma (C) 2010 Elsevier Ltd All rights reserved

JOURNAL OF INSECT PHYSIOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

56

12

1889

1894

10.1016/j.jinsphys.2010.08.008

https://doi.org/10.1016/j.jinsphys.2010.08.008
https://www.ncbi.nlm.nih.gov/pubmed/20713057
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000284568800023&DestApp=WOS_CPL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77958484557&origin=inward
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77958484557&origin=inward