Academic Thesis

Basic information

Name Katayama Keiichi
Belonging department
Occupation name
researchmap researcher code B000305947
researchmap agency Okayama University of Science

Title

Induction of cytochrome P450 isozymes by phenobarbital in pregnant rat and fetal livers and placenta

Bibliography Type

 

Author

N Ejiri
K Katayama
K Doi

Summary

Cytochrome P450 (CYP) isozymes are important in metabolizing xenobiotics. They are found in extrahepatic tissues such as placenta as well as liver. Previously, we reported that CYP3A1 was detected in the cytoplasm of giant cells in the trophoblastic region of placenta of rats through pregnancy. In this study, we examined the changes in the expression of CYP proteins in the pregnant rat and fetal livers and placenta after treatment with phenobarbital (PB), one of the antiepileptic drugs which is well known to induce several phase I and phase 11 drug metabolizing enzymes in the liver. Namely, F344 pregnant rats were treated with PB (80 mg/kg, i.p.) from 13 days of gestation (DG) to 16 DG. All animals were sacrificed on 17 DG, and Western blot analysis and ii-nmunohistochemical staining on nine CYP proteins (CYP1A1, CYP2B1, CYP2C6, CYP2C12, CYP2D1, CYP2D4, CYP2E1, CYP3A1, and CYP4A1) and histological examination were done in the dam's liver, placenta, and the fetal liver. Western blot analysis revealed that CYP3A1 protein was significantly induced, CYP2B1 protein was detected, and CYP2D1 protein was significantly decreased in the dam's liver after PB-treatment. In placenta, only CYP3A1 was detected with no difference between control and PB-treated animals, The results of immunohistochemical staining corresponded closely to those of Western blot analysis in the dam's liver and placenta. In the fetal liver. CYP3A1 and CYP2C6 proteins were significantly induced after the PB-treatment, but their immunostainability was not prominent. The present results are considered useful as a basis for further investigation of drug metabolism in pregnant animals. (c) 2004 Elsevier Inc. All rights reserved.

Magazine(name)

EXPERIMENTAL AND MOLECULAR PATHOLOGY

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE

Volume

78

Number Of Pages

2

StartingPage

150

EndingPage

155

Date of Issue

2005-04

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

 

DOI

10.1016/j.yexmp.2004.07.002

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PMID

 

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arXiv ID

 

ORCID Put Code

 

DBLP ID