Academic Thesis

Basic information

Name Katayama Keiichi
Belonging department
Occupation name
researchmap researcher code B000305947
researchmap agency Okayama University of Science

Title

5-azacytidine (5AzC)-induced histopathological changes in the central nervous system of rat fetuses

Bibliography Type

 

Author

M Ueno
K Katayama
A Yasoshima
H Nakayama
K Doi

Summary

5-Azacytidine (5AzC) is a cytidine analogue which possesses nitrogen atom instead of carbon atom at the position 5 of the pyrimidine ring. In this study, detailed histopathological changes were sequentially examined in the rat fetal brain obtained from dams treated with 5AzC (10 mg/kg) on day 13 of gestation (GD13). At 6 hours after treatment (HAT), a prominent accumulation of neuroepithelial cells showing pleomorphic mitotic figures were observed in the telencephalic wall. The mitosis-index peaked at 6 HAT, and decreased thereafter. Neuroepithelial cells positive for nick end labeling (TUNEL) method, which is widely used for the detection of apoptotic cells, prominently increased at 9 HAT, and the TUNEL-index peaked at 12 HAT. TUNEL-positive cells showed ultrastructural characteristics of apoptosis. At 24 HAT, the formation of rosette-like structures was observed in the fetal brain. From the results of the present study, it was evident that abnormal mitosis and neuronal apoptosis were induced in the rat fetal brain following 5AzC-administration to dams on GD13. In addition, it is suggested that 5AzC-induced apoptosis might occur mainly in the post mitotic phase of cell cycle.

Magazine(name)

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY

Publisher

URBAN & FISCHER VERLAG

Volume

54

Number Of Pages

2

StartingPage

91

EndingPage

96

Date of Issue

2002-08

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

 

DOI

10.1078/0940-2993-00239

NAID

 

PMID

 

J-GLOBAL ID

 

arXiv ID

 

ORCID Put Code

 

DBLP ID