Academic Thesis

Basic information

Name Katayama Keiichi
Belonging department
Occupation name
researchmap researcher code B000305947
researchmap agency Okayama University of Science

Title

Development of apoptosis and changes in apoptosis-related genes expression in the thymus of nivalenol-treated mice

Bibliography Type

 

Author

Amnart Poapolathep
Kazuhiko Suzuki
Kei-Ichi Katayama
Ryoichi Ohtsuka
Takayuki Nagata
Koji Uetsuka
Hiroyuki Nakayama
Kunio Doi

Summary

Nivalenol (NIV) is a potent toxic trichothecene mycotoxin produced by Fusarium nivale, and our previous study clarified first that NIV induces apoptosis in lymphoid organs of mice in a dose-dependent manner. In this study, the development of apoptosis and changes in the expression of apoptosis-related genes (fas, c-fos, c-jun, p53, bcl-2 and c-myc) mRNAs were examined in the thymus of mice for up to 12 hours after inoculation (HAI) of NIV (15 mg/kg b.w.) to elucidate the relationship between the molecular genetic regulatory mechanisms and NIV-induced lymphocyte apoptosis. The number of apoptotic lymphocytes evaluated by TUNEL method clearly increased from 3 HAI and peaked at 9 HAL The DNA ladder formation by agarose gel electrophoresis was clearly observed at 6 and 9 HAL The expression of c-fos and c-jun mRNAs clearly elevated from 0.5 HAI and peaked at 1 HAL The expression of fas, p53, bcl-2 and c-myc mRNAs showed no significant changes. These results indicate that c-fos and c-jun may play an important role in NIV- induced lymphocyte apoptosis.

Magazine(name)

Journal of Toxicologic Pathology

Publisher

Japanese Society of Toxicologic Pathology

Volume

14

Number Of Pages

4

StartingPage

299

EndingPage

304

Date of Issue

2001

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

 

DOI

10.1293/tox.14.299

NAID

 

PMID

 

J-GLOBAL ID

 

arXiv ID

 

ORCID Put Code

 

DBLP ID