Academic Thesis

Basic information

Name Higashi Tsunehito
Belonging department
Occupation name
researchmap researcher code 5000085325
researchmap agency Okayama University of Science

Title

Cystine transporter SLC7A11 regulates sensitivity to unsaturated carbonyl compounds in mouse macrophage cell lines

Bibliography Type

Joint Author

Author

Tsunehito Higashi Konoka Hashimoto Yosuke Mai Fumito Naganuma Takeo Yoshikawa

Summary

Cytotoxic effects of cigarette smoke are thought to be causes of cigarette smoking-related diseases such as respiratory infection, chronic obstructive pulmonary disease, and atherosclerosis. Unsaturated carbonyl compounds are major cytotoxic factors in the gas phase of cigarette smoke. Cell death induced by unsaturated carbonyl compounds in cigarette smoke is PKC-dependent ferroptosis. Although the cell sensitivity to unsaturated carbonyl compounds varies by cell types, the molecular mechanisms underlying this sensitivity remain unclear. In this study, we have examined the factors involved in determining sensitivity to unsaturated carbonyl compounds. We found that two mouse macrophage cell lines exhibited different sensitivities; J774.1 macrophages were sensitive to unsaturated carbonyl compounds, whereas RAW264.7 macrophages were resistant. Glutathione synthesis inhibitor increased the sensitivity of RAW264.7 macrophages to unsaturated carbonyl compounds. Quantitative RT-PCR revealed that the expression level of the cystine transporter SLC7A11 was higher in RAW264.7 macrophages than in J774.1 macrophages. Inhibition of SLC7A11 activity increased sensitivity to unsaturated carbonyl compounds, while overexpression of SLC7A11 enhances resistance to these compounds. The current results suggest that the SLC7A11 level is a key factor in determining the macrophage sensitivity to unsaturated carbonyl compounds.

Magazine(name)

Journal of Pharmacological Sciences

Publisher

Elsevier BV

Volume

157

Number Of Pages

2

StartingPage

96

EndingPage

103

Date of Issue

2025/02

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

DOI

10.1016/j.jphs.2024.12.008

NAID

PMID

J-GLOBAL ID

arXiv ID

ORCID Put Code

DBLP ID