Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator

T Shimada
W Watanabe
S Matsunaga
T Higashi
H Ishii
K Fukui
K Isobe
K Itoh

Femtosecond laser pulses can be used to selectively disrupt and dissect intracellular organelles. We report on disruption of mitochondria in living HeLa cells using a femtosecond laser oscillator with a repetition rate of 76 MHz. We studied the laser parameters used for disruption. The long-term viability of the cells after disruption of a single mitochondrion was confirmed by the observation of cell division, indicating that intracellular disruption of organelles using a femtosecond laser oscillator can be performed without compromising the long-term cell viability. (c) 2005 Optical Society of America

OPTICS EXPRESS

OPTICAL SOC AMER

13

24

9869

9880

10.1364/OPEX.13.009869

https://doi.org/10.1364/OPEX.13.009869
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