Multi-spectral two-photon excited fluorescence microscopy using supercontinuum light source

K Isobe
W Watanabe
S Matsunaga
T Higashi
K Fukui
K Itoh

We report on a novel technique of multi-spectral two-photon-excited fluorescence microscopy by use of broadband supercontinuum light. A supercontinuum in the near-infrared region is generated in a 4.5-min-long photonic crystal fiber using a Ti:sapphire femtosecond oscillator. The supercontinuum is used as a multi-spectral excitation light source in a two-photon excited fluorescence microscope. We demonstrate that three-color fluorescence images of organelles in a cell can be simultaneously acquired.

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS

INST PURE APPLIED PHYSICS

44

1-7

L167

L169

10.1143/JJAP.44.L167

http://ci.nii.ac.jp/naid/150000013358
https://doi.org/10.1143/JJAP.44.L167
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000227675600054&DestApp=WOS_CPL