Femtosecond laser disruption of subcellular organelles in a living cell

W Watanabe
N Arakawa
S Matsunaga
T Higashi
K Fukui
K Isobe
K Itoh

Subcellular organelles in living cells were inactivated by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of a mitochondrion in living cells was experimentally confirmed by stacking three-dimensional confocal images and by restaining of organelles. The viability of the cells after femtosecond laser irradiation was ascertained by impermeability of propidium iodide as well as by the presence of cytoplasmic streaming. (C) 2004 Optical Society of America.

OPTICS EXPRESS

OPTICAL SOC AMER

12

18

4203

4213

10.1364/OPEX.12.004203

https://doi.org/10.1364/OPEX.12.004203
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000223723300005&DestApp=WOS_CPL