An Arabidopsis thaliana gene on the yeast artificial chromosome can be transcribed in tobacco cells

Haibo Liu
Akira Kawabe
Sachihiro Matsunaga
Hee Kim Yeon
Tsunehito Higashi
Susumu Uchiyama
Satoshi Harashima
Akio Kobayashi
Kiichi Fukui

A part of chromosome 5 from Arabidopsis thaliana, which has 36 genes, on yeast artificial chromosome (YAC) was transferred into tobacco BY-2 cells using a bio-active beads method. The YAC was constructed using the chromosome-splitting technique. The GFP gene located on the YAC showed repeated transient expression after transformation. Molecular analyses confirmed that 1 (At5g57980) of the 5 genes checked had transcribed in the tobacco BY-2 cells. This demonstrates that the promoter of this gene could work in both A. thaliana and tobacco BY-2 cells.

Cytologia

69

2

235

240

10.1508/cytologia.69.235

https://doi.org/10.1508/cytologia.69.235
http://ci.nii.ac.jp/naid/10012803229
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