【Introduction】Bladder cancer (BC) is the most common neoplasmaffecting the urinary tract of dogs. Stem cell-derived 3D organoid culture couldrecapitulate organ structure and physiology. In our previous study, urinesample-derived dog prostate cancer organoids have been established. However,urine-derived dog BC organoids have never been developed. We therefore generateddog BC organoids using the urine samples.
【Methods and Results】After dogs were clinically diagnosed withbladder tumor, urine samples were collected by catheterization and used for theorganoid culture. Organoids from each BC dog were successfully generated.Expression of an epithelial cell marker (E-cadherin) and a myofibroblast marker(α-smooth muscle actin, (SMA)) was confirmed in the organoids. The organoidsalso expressed a stem cell marker (CD44). Injection of the BC organoids intoimmunodeficiency mice successfully generated tumor. While treatment withcisplatin and vinblastine decreased cell viability of organoids in adose-dependent manner, treatment with gemcitabine and piroxicam had littleeffect on the cell viability of the organoids.
【Conclusions】These findings revealed that BC organoids derived fromurine stem cells might become a useful tool to investigate the mechanisms ofpathogenesis and treatment of dog BC
