Academic Thesis

Basic information

Name Mitsui Ryoji
Belonging department
Occupation name
researchmap researcher code 1000364532
researchmap agency Okayama University of Science

Title

Organization of the genes involved in the ribulose monophosphate pathway in an obligate methylotrophic bacterium, Methylomonas aminofaciens 77a.

Bibliography Type

Author

Yasuyoshi Sakai, Ryoji Mitsui, Yumiko Katayama, Hideshi Yanase, Nobuo Kato.

Summary

Methylotroph, Methylomonas, Ribulose monophosphate pathway
he 4.4-kb PstI fragment harboring the gene encoding 3-hexulose-6-phosphate synthase, rmpA, which was previously cloned from the chromosome of an obligate methylotroph, Methylomonas aminofaciens 77a, was investigated in detail. In addition to the rmpA gene, the fragment contained three open reading frames encoding transaldolase (rmpD), IS10-R (rmpI), and 6-phospho-3-hexuloisomerase (PHI) (rmpB). The rmpB gene product was overproduced in Escherichia coli cells, purified to homogeneity, and then enzymatically identified as PHI. The gene organization of the ribulose monophosphate pathway enzymes together with a transposon, IS10-R, is discussed from both evolutionary and regulatory aspects.


Magazine(name)

FEMS Microbiol Letter

Publisher

ELSEVIER SCIENCE

Volume

Number Of Pages

StartingPage

EndingPage

Date of Issue

1999/07

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

DOI

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PMID

URL

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