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Glycosylation of Piceid and Resveratroloside by Bioconversion with Phytolacca americana Glucosyltransferase Expressed in Escherichia coli
Kei Shimoda, Naoji Kubota, Shouta Okada, Shouta Doi, Daisuke Uesugi, Hatsuyuki Hamada, Hiroki Hamada, Atsuhito Kuboki, Takafumi Iwaki, Yuya Kiriake, Tomohiro Saikawa, Kohji Ishihara
The glycosylation of piceid and resveratroloside was investigated using glucosyltransferase from Phytolacca americana expressed in Escherichia coli. The enzyme glucosylated piceid at its 5- and 4′-positions to give resveratrol 3,5-diglucoside and resveratrol 3,4′-diglucoside, resp. Resveratroloside was glucosylated to resveratrol 3,4′-diglucoside by the enzyme. |