Preparation of Paclitaxel Glycoside-Anioinic Nanoparticles, Curcumin Gluco-oligosaccharides, alpha-Tocopherol Glycoside, Daizein Glycoside, and Genistein Glycoside and their Application for Treatment of Skin Cancer, Dementia, and Allergy

H. Hamada, D. Uesugi, K. Ishihara, R. Hosoda, K. Shimoda, A. Kuboki, and Y. Kiriake

Biotransformation is a useful tool for preparation of biomedically active compounds. This study reports the preparation of glycoside compounds and their medical applications. Composite nanoparticles, “anionic liposomes”, composed of anionic Technol PG and paclitaxel glycoside were prepared by mixing them in water with cholic acid-based surfactants of SC and a subsequent heating/cooling/ultrasonicating process. Small-sized anionic Technol PG nanoparticles (particle size: 3 nm) could be prepared by ultrasonic fragmentation at low temperature of 4°C. Upon addition of paclitaxel glycoside-anionic Technol PG nanoparticles to rat skin tissue (in vitro), the nanoparticles “anionic liposomes” (particle size: 3 nm) infiltrated into the epidermis layer penetrating stratum corneum (intercellular space: ca. 100 nm). In addition, during the anti-skin cancer test using mouse model of skin cancer, our study revealed that the numbers of papillomas of the mouse applied with paclitaxel glycoside-anionic Technol PG nanoparticles, “anionic liposomes”, to mouse skin were decreased, although those of the mouse applied with paclitaxel glycoside itself to mouse skin (control) were increased. Thus, this study established that since paclitaxel glycoside-anionic Technol PG nanoparticles could permeate stratum corneum and be incorporated into the epidermis layer of mouse, they could also treat skin cancer (in vivo). On the other hand, biotransformation of curcumin was achieved by using enzymes as biocatalysts. Curcumin gluco-oligosaccharides, which were intraperitoneally or orally injected to a mouse, could penetrate the BBB of mouse brain and be incorporated into the mouse’s brain tissue, and could enhance spatial learning (in vivo). In addition, glucoside and galactoside of α-tocopherol showed high anti-allergic activity toward allergen, glutenin. Also, glucoside and galactoside of daidzein and genistein had strong anti-allergic activity against allergen, globulin.

International Journal of Current Microbiology and Applied Sciences

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https//doi.org/10.20546/ijcmas.2024.1307.xx