Comparison of Analytical Values after Changing to the International Standardized Method for Lactate Dehydrogenase and Alkaline Phosphatase Measurements in Mouse and Rat

Kayo Furumoto,Noboru Fujitani ,Masakatsu Nohara, Akihisa Hata

Since April 2020 in Japan, lactate dehydrogenase and alkaline phosphatase assays, which were earlier conducted using the Japan Society of Clinical Chemistry protocols, are being conducted using the International Federation of Clinical Chemistry and Laboratory Medicine protocols. The correlation between blood values measured by these methods is not known in some species; therefore, values measured using these two methods cannot be used interchangeably. In this study, the relationship between lactate dehydrogenase and alkaline phosphatase values of mice and rats using each method was determined, and coefficients were generated to convert and compare the obtained values. These coefficients can be used for the mutual conversion of measured values during the transition period from the Japan Society of Clinical Chemistry method to the International Federation of Clinical Chemistry and Laboratory Medicine method. However, it should be noted that the conversion coefficients were affected by isozyme composition.

Veterinary Science

9

10.3390/vetsci9110595