Academic Thesis

Basic information

Name Maeta Noritaka
Belonging department
Occupation name
researchmap researcher code B000312461
researchmap agency Okayama University of Science

Title

 Comparative analysis of canine mesenchymal stem cells and bone marrow-derived mononuclear cells.

Bibliography Type

Joint Author

Author

Noritaka Maeta, Katsutoshi Tamura, Fuuna Ezuka and Hiroshi Takemitsu

Summary

Background and aim:Mesenchymal stem cells (MSCs), which have multi-lineage differentiation potentials, are a promising source for regenerative medicine. However, the focus of study of MSCs is shifting from the characterization of the differentiation potential to their secretion potential for cell transplantation. Tissue regeneration and the attenuation of immune responses are thought to be affected by the secretion of multiple growth factors and cytokines by MSCs. However, the secretion potential of MSCs profiling remains incompletely characterized. In this study, we focused on the secretion ability related and protein mRNA expression of dog adipose tissue-derived MSCs (AT-MSC), bone marrow (BM)-derived MSCs, and BM-derived mononuclear cells (BM-MNC).    
Materials and methods:Real-time polymerase chain reaction analyses revealed mRNA expression of nine growth factors and seven interleukins in these types of cells and three growth factors protein expression were determined using Enzyme-linked immunosorbent assay.    
Results:For the BM-MNC growth factors, the mRNA expression of transforming growth factor-β (TGF-β) was the highest. For the BM-derived MSC (BM-MSC) and AT-MSC growth factors, the mRNA expression of vascular endothelial growth factor (VEGF) was highest. BM-MSCs and AT-MSCs showed similar expression profiles. In contrast, BM-MNCs showed unique expression profiles for hepatocyte growth factor and epidermal growth factor. The three types of cells showed a similar expression of TGF-β.    
Conclusion:We conclude that expression of cytokine proteins and mRNAs suggests involvement in tissue repair and protection.    

Magazine(name)

Vet world.

Publisher

Volume

14

Number Of Pages

4

StartingPage

1028

EndingPage

1037

Date of Issue

2021/04

Referee

Exist

Invited

Not exist

Language

Thesis Type

ISSN

DOI

doi: 10.14202/vetworld.2021.1028-1037.

NAID

PMID

URL

J-GLOBAL ID

arXiv ID

ORCID Put Code

DBLP ID