Academic Thesis

Basic information

Name Saeki Kohei
Belonging department
Occupation name
researchmap researcher code R000007576
researchmap agency Okayama University of Science

Title

Detection of human epidermal growth factor receptor 2 overexpression in canine anal sac gland carcinoma.

Bibliography Type

Author

[Sho Yoshimoto,Daiki Kato,Satoshi Kamoto,Kie Yamamoto,Masaya Tsuboi,Masahiro Shinada,Namiko Ikeda,Yuiko Tanaka,Ryohei Yoshitake,Shotaro Eto,Kohei Saeki,James Kenn Chambers,Ryohei Kinoshita,Kazuyuki Uchida,Ryohei Nishimura,Takayuki Nakagawa]

Summary

Canine anal sac gland carcinoma (ASGC) frequently occurs in the apocrine glands of the canine anal sac and shows aggressive biological behavior. The expression of human epidermal growth factor receptor 2 (HER2) has been reported in various human and canine tumors. HER2 is a promising therapeutic target of these tumors, and HER2-targeted drugs, such as trastuzumab and lapatinib, have improved the outcome of these patients. In this study, HER2 expression in ASGC was evaluated to investigate its potential as a therapeutic target for canine ASGC. HER2 mRNA expression in surgically resected ASGC tissues was significantly higher than that in normal anal sac tissue. To evaluate the expression of HER2 protein, paraffin-embedded ASGC tissues were immunohistochemically evaluated. Strong and broad staining of HER2 was detected in ASGC tissues, while HER2 was weakly to moderately stained in normal anal sac apocrine glands and squamous epithelia. The degree of HER2 expression in ASGC tissues was scored based on its intensity and positivity (score: 0-3+). Scoring of HER2 expression revealed 6 samples (24%) scored 3+, 14 (56%) scored 2+, and 5 (20%) scored 1+, with no samples scoring 0. In all, 80% of canine ASGC tissues were positive for HER2 (scored ?2+). Furthermore, putative HER2-overexpressed cells in ASGC were detected with trastuzumab by flow cytometry. These preliminary data may lead to further evaluation of the role of HER2 in canine ASGC as a mechanism of malignancy and as a therapeutic target for HER2-targeted therapy.


Magazine(name)

The Journal of veterinary medical science

Publisher

Volume

81

Number Of Pages

7

StartingPage

1034

EndingPage

1039

Date of Issue

2019/07

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

DOI

NAID

PMID

URL

J-GLOBAL ID

arXiv ID

ORCID Put Code

DBLP ID