Microfluidic preparation of anchored cell membrane sheets for in vitro analyses and manipulation of the cytoplasmic face

Izuta,S., Yamaguchi,S., Misawa,R., Yamahira,S., Tan,M., Kawahara,M., Suzuki,T., Takagi,T., Sato,K., Nakamura,M., Nagamune,T. and Okamoto,A.

Abstract: A microfluidic method to prepare cell membrane sheets was developed as a
promising tool for direct examination of the cytoplasmic faces of cellular plasma
membranes. Mammalian cells immobilized on a poly(ethylene glycol)-lipid coated
substrate were rapidly and efficiently fractured, with the sheer stress of laminar flow in
microchannels, resulting in isolation of the bottom cell membrane sheets with exposed
intact cytoplasmic faces. On these faces of the cell membrane sheets, both ligand-
induced phosphorylation of receptor tyrosine kinases and selective enzymatic
modification of a G-protein coupling receptor were directly observed.Microfluidic preparation of anchored cell membrane sheets for in vitro analyses and manipulation of the cytoplasmic face

Sci. Rep.