Molecular Epidemiological Survey of the Babesia gibsoni cytochrome b Gene in Western Japan

Masato Sakuma
Kenta Fukuda
Katsuyoshi Takayama
Yukuharu Kobayashi
Takako Shimokawa Miyama
Asuka Setoguchi
Yasuyuki Endo

In this study, we conducted a survey of the cytochrome b (cytb) gene of Babesia gibsoni (B. gibsoni) isolated from clinical cases to determine the prevalence of potential atovaquone (ATV)-resistant variants. Ninety-two blood samples were collected from naturally B. gibsoni infected dogs. The cytb nucleotide sequence was determined by direct sequencing. Twelve non-synonymous amino acid substitutions were identified in cytb. The principal ATV-resistant substitution, M1211, was detected in three cases. This survey determined that potentially ATV-resistant B. gibsoni strains are present in dogs in Japan.

JOURNAL OF VETERINARY MEDICAL SCIENCE

JAPAN SOC VET SCI

74

10

1341

1344

10.1292/jvms.12-0140

https://www.ncbi.nlm.nih.gov/pubmed/22672842
https://doi.org/10.1292/jvms.12-0140
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000311593600017&DestApp=WOS_CPL