Molecular Cloning of the Canine Fragile Histidine Triad (FHIT) Gene and Fhit Protein Expression in Canine Peripheral Blood Mononuclear Cells

Hiroko Hiraoka
Koji Minami
Naoki Kaneko
Takako Shimokawa Miyama
Takuya Mizuno
Masaru Okuda

A fragile histidine triad (FHIT) gene has been studied as a tumor-associated gene in humans. The aberrant FHIT gene and its protein expression have been reported in many types of human cancers. The present Study explored the canine FHIT gene structure and its protein expression in the peripheral blood mononuclear cells of healthy dogs by RT-PCR, RACE and immunobolt analysis. The obtained canine FHIT gene contained nine small exons and was located on canine chromosome 20. Furthermore, we identified in alternative splicing form of file FHIT transcript. The deduced amino acid sequence was well conserved between species, and anti-human Fhit antibody could be used to detect the canine Fhit protein. These findings will be useful for future research.

JOURNAL OF VETERINARY MEDICAL SCIENCE

JAPAN SOC VET SCI

71

5

645

649

https://www.ncbi.nlm.nih.gov/pubmed/19498293
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