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| Basic information |
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| Name |
Miyabe Masahiro |
| Belonging department |
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| Occupation name |
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| researchmap researcher code |
R000000646 |
| researchmap agency |
Okayama University of Science |
Establishment of a canine lens epithelial cell line.
Akira Matsuda, Yuki Shimizu, Teppei Kanda, Akihiro Ohnishi, Noritaka Maeta, Masahiro Miyabe, Kaori Saeki, Yoshiki Itoh
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Some immortalized lens epithelial cell lines have been established and are useful for molecular analysis. The establishment of additional cell lines must, however, enable a variety of in-vitroexaminations. The objective of this study was to establish a new canine lens epithelial cell line by isolating CLC-1 cells from the lens tissue of a dog with cataracts. In CLC-1 cells, transforming growth factor beta (TGF-β) treatment significantly decreased gene expression of an epithelial marker and elevated that of mesenchymal markers; these characteristics are similar to those of a human lens epithelial cell line. Interestingly, CLC-1 cells exhibited lower expression of an epithelial marker and higher expression of mesenchymal markers than an anterior lens capsule. These results suggest that CLC-1 cells were derived from a cell population that was committed to epithelial-mesenchymal transition in cataract lens tissue. In conclusion, CLC-1 cells could be useful for analyzing molecular pathogenesis in canine cataracts.
Canadian Journal of veterinary research
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