論文

基本情報

氏名 山本 直樹
氏名(カナ) ヤマモト ナオキ
氏名(英語) Yamamoto Naoki
所属 獣医学部 獣医学科
職名 講師
researchmap研究者コード R000065967
researchmap機関 岡山理科大学

題名

Lipopolysaccharide (LPS) suppresses follicle development marker expression and enhances cytokine expressions, which results in fail to granulosa cell proliferation in developing follicle in cows.

単著・共著の別

 

著者

Naoki Yamamoto
Himeno Takeuchi
Manami Yamaoka
Tomoya Nakanishi
Shingo Tonai
Ryo Nishimura
Takehito Morita
Masashi Nagano
Shingo Kameda
Kaori Genda
Jun Kawase
Yasuhisa Yamashita

概要

Postpartum endometritis is known to be associated with ovarian dysfunction in cows. Lipopolysaccharide (LPS) generated by Gram-negative bacteria is recognized by toll-like receptor 4 (TLR4), which leads to an inflammatory response by the generation of cytokines such as tumor necrosis factor-α (TNF-α) and interleukins. In this study, we investigated the effect of endometrial LPS on granulosa cell functions during early follicular development in cows. Uteri and follicles were obtained from a slaughterhouse and classified into either clinical endometritis (CE) or normal groups by vaginal mucus test. TLR4 mRNA and protein in normal cows were expressed in granulosa cells collected from follicles measuring 1-3 and 4-7 mm in a diameter, respectively. LPS content in endometrium and follicular fluid of CE cows was significantly higher than that in normal cows. Compared to normal cows, CE cows showed lower expression of follicular development markers (FSHR, CYP19A1, CCND2, and LHCGR) in granulosa cells, lower estradiol-17β concentrations in follicular fluid, and lower granulosa cell proliferation. CE contraction significantly increased cytokine expressions (TNF, IL-1A, and IL-1B) in granulosa cells and suppressed apoptosis of granulosa cells compared to normal cows. LPS significantly suppressed the expression of follicular development markers and the production of estradiol-17β in granulosa cells and reduced granulosa cells proliferation compared to cells cultured without LPS. LPS significantly increased cytokine expressions and suppressed granulosa cell apoptosis. Thus, the present results suggest that the existence of LPS in developing follicles is one of the causes of ovarian quiescence in cows.

発表雑誌等の名称

Reproductive biology

出版者

 

23

1

開始ページ

100710

終了ページ

100710

発行又は発表の年月

2023-03

査読の有無

 

招待の有無

 

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

 

ID:DOI

10.1016/j.repbio.2022.100710

ID:NAID(CiNiiのID)

 

ID:PMID

 

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID