論文

基本情報

氏名 東 恒仁
氏名(カナ) ヒガシ ツネヒト
氏名(英語) Higashi Tsunehito
所属 獣医学部 獣医学科
職名 准教授
researchmap研究者コード 5000085325
researchmap機関 岡山理科大学

題名

Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse

単著・共著の別

 

著者

T Higashi
S Miyakawa
S Uchiyama
S Matsunaga
H Takata
S Fujimoto
M Noda
A Terauchi
T Shimizu
M Oda
T Azuma
K Fukui

概要

We raised monoclonal antibodies by immunizing mice with total chromosome proteins extracted from isolated human metaphase chromosomes. The indirect immunofluorescence, screening of hybridoma cell lines provided 15 monoclonal antibodies against the chromosomal antigens. The antigen proteins of the mAbs were identified by immunoblotting as core histones or by immunoprecipitation followed by a peptide mass fingerprinting method as nuclear mitotic apparatus protein, heterogeneous nuclear ribonucleoprotein A2/B1, ribosomal protein S4, linker histone and beta-actin. During mitosis, localizations of these proteins on chromosomes were clearly observed using the obtained antibodies. These results indicate that the current strategy is effective for obtaining monoclonal antibodies useful for immunoblotting and/or immunofluorescent staining of human proteins, using the antigens with high homology to mouse proteins. (c) 2005 Elsevier B.V. All rights reserved.

発表雑誌等の名称

JOURNAL OF BIOTECHNOLOGY

出版者

ELSEVIER SCIENCE BV

120

3

開始ページ

262

終了ページ

272

発行又は発表の年月

2005-11

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

 

ID:DOI

10.1016/j.jbiotec.2005.06.036

ID:NAID(CiNiiのID)

 

ID:PMID

 

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID