論文

基本情報

氏名 東 恒仁
氏名(カナ) ヒガシ ツネヒト
氏名(英語) Higashi Tsunehito
所属 獣医学部 獣医学科
職名 准教授
researchmap研究者コード 5000085325
researchmap機関 岡山理科大学

題名

Proteome analysis of human metaphase chromosomes

単著・共著の別

 

著者

S Uchiyama
S Kobayashi
H Takata
T Ishihara
N Hori
T Higashi
K Hayashihara
T Sone
D Higo
T Nirasawa
T Takao
S Matsunaga
K Fukui

概要

DNA is packaged as chromatin in the interphase nucleus. During mitosis, chromatin fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal DNA into the daughter cells. Despite > 1 century of research on metaphase chromosomes, information regarding the higher order structure of metaphase chromosomes is limited, and it is still not clear which proteins are involved in further folding of the chromatin fiber into metaphase chromosomes. To obtain a global view of the chromosomal proteins, we performed proteome analyses on three types of isolated human metaphase chromosomes. We first show the results from comparative proteome analyses of two types of isolated human metaphase chromosomes that have been frequently used in biochemical and morphological analyses. 209 proteins were quantitatively identified and classified into six groups on the basis of their known interphase localization. Furthermore, a list of 107 proteins was obtained from the proteome analyses of highly purified metaphase chromosomes, the majority of which are essential for chromosome structure and function. Based on the information obtained on these proteins and on their localizations during mitosis as assessed by immunostaining, we present a four-layer model of metaphase chromosomes. According to this model, the chromosomal proteins have been newly classified into each of four groups: chromosome coating proteins, chromosome peripheral proteins, chromosome structural proteins, and chromosome fibrous proteins. This analysis represents the first compositional view of human metaphase chromosomes and provides a protein framework for future research on this topic.

発表雑誌等の名称

JOURNAL OF BIOLOGICAL CHEMISTRY

出版者

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

280

17

開始ページ

16994

終了ページ

17004

発行又は発表の年月

2005-04

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

 

ID:DOI

10.1074/jbc.M412774200

ID:NAID(CiNiiのID)

 

ID:PMID

 

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID