論文

基本情報

氏名 東 恒仁
氏名(カナ) ヒガシ ツネヒト
氏名(英語) Higashi Tsunehito
所属 獣医学部 獣医学科
職名 准教授
researchmap研究者コード 5000085325
researchmap機関 岡山理科大学

題名

A novel transfection method for mammalian cells using calcium alginate microbeads

単著・共著の別

 

著者

T Higashi
E Nagamori
T Sone
S Matsunaga
K Fukui

概要

The direct transfer of genetic materials into mammalian cells is an indispensable technique. We have developed calcium alginate (CA) microbeads which can deliver plasmid DNAs and yeast artificial chromosomes into plant and yeast cells. In this paper, we demonstrate the effective transfection of mammalian cells by CA microbeads immobilizing plasmid DNAs. The transfection was performed using the pEGFP-C1 plasmid containing the cytomegalovirus (CMV) promoter and enhanced green fluorescent protein (EGFP) gene. The transient expression of EGFP was observed 24 h after transfection. The expression efficiency was maximum when the concentration of sodium alginate was 1% and the amount of plasmid DNA was increased to 100 mug. The expression efficiency of our method using CA microbeads is 2-10 times higher than that of the polyethylene glycol (PEG) method. Our results suggest that the CA microbead mediated transfection of mammalian cells effectively delivers genetic materials into mammalian suspension cells.

発表雑誌等の名称

JOURNAL OF BIOSCIENCE AND BIOENGINEERING

出版者

SOC BIOSCIENCE BIOENGINEERING JAPAN

97

3

開始ページ

191

終了ページ

195

発行又は発表の年月

2004-03

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

 

ID:DOI

10.1263/jbb.97.191

ID:NAID(CiNiiのID)

 

ID:PMID

 

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID