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| 基本情報 |
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| 氏名 |
池田 正五 |
| 氏名（カナ） |
ｲｹﾀﾞ ｼﾖｳｺﾞ |
| 氏名（英語） |
Ikeda Shiyogo |
| 所属 |
生命科学部 生物科学科 |
| 職名 |
教授 |
| researchmap研究者コード |
1000113908 |
| researchmap機関 |
岡山理科大学 |
Rapid purification of squirrel monkey retrovirus-H major gag protein by high performance liquid chromatography.
Ikeda S, Tsutsui K, Hatsushika M, Watanabe S, Oda T.
The major gag protein (p34) of squirrel monkey retrovirus-H was purified in one chromatographic step by anion-exchange high performance liquid chromatography. The virus in a crude fraction was disrupted with Brij 35 in the presence of three kinds of protease inhibitors. The soluble virus lysate was injected into a Polyanion SI column, and p34 was eluted with a linear salt gradient. The recovery of the protein was about 60%. The purified p34 was nearly homogenous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining.
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