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| 基本情報 |
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| 氏名 |
池田 正五 |
| 氏名（カナ） |
ｲｹﾀﾞ ｼﾖｳｺﾞ |
| 氏名（英語） |
Ikeda Shiyogo |
| 所属 |
生命科学部 生物科学科 |
| 職名 |
教授 |
| researchmap研究者コード |
1000113908 |
| researchmap機関 |
岡山理科大学 |
Expression of simian virus 40 small t antigen in Escherichia coli and purification of the antigen.
Ikeda S, Akiyama K, Mitsunobu F, Wada T, Hatsushika M, Watanabe S, Tsutsui K, Seki S, Oda T.
A simian virus 40 (SV 40) DNA fragment encoding small t antigen was cloned in expression vector pUC8 for the purification of the antigen. The SV40 Hind III B fragment was inserted into the Hind III site of pUC8. A plasmid having the lacZ' and small t antigen genes in the same orientation was designated as pSVt. pSVt encodes the entire small t antigen and an extra 18 amino acids at the amino terminus of the antigen. E. coli transformed with pSVt produced hybrid small t antigen (22 kDa) which comprised about 6% of the total protein. The hybrid small t antigen reacted in immunoblot analysis with SV40-induced tumor-bearing hamster serum. Hybrid small t antigen was extracted from E. coli, and purified by preparative SDS-PAGE. The antigen was extracted from the gel using formic acid solution with high-yield. The gel-purified antigen showed the same antigenic reactivity as crude antigen.
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