論文

基本情報

氏名 池田 正五
氏名(カナ) イケダ シヨウゴ
氏名(英語) Ikeda Shiyogo
所属 生命科学部 生物科学科
職名 教授
researchmap研究者コード 1000113908
researchmap機関 岡山理科大学

題名

Base Excision Repair of Oxidative DNA Damage in a Catalase-deficient Mutant of Schizosaccharomyces pombe

単著・共著の別

著者

Hida, Y and Ikeda, S.

概要

Base excision repair (BER) is the major pathway to repair oxidized bases in many organisms, but the BER mutants of Schizosaccharomyces pombe are substantially resistant to hydrogen peroxide.  To reduce the reactive oxygen species (ROS) scavenging activity in cells, we disrupted a catalase gene, ctt1, in S. pombe.  The ctt1 mutant became sensitive to hydrogen peroxide, but had no mutator phenotype.  Deletion of the BER genes (nth1, apn1, apn2, or uve1) from ctt1 mutant further increased the hydrogen peroxide sensitivity, indicating that the catalase activity obscures the functions of BER enzymes in vivo.  The nth1 and apn2 mutants exhibited a moderate mutator phenotype.  Double mutants in both ctt1 and BER genes showed extremely high spontaneous mutation rates, especially in the ctt1/nth1 mutant.  Vitamin C relieved the mutator phenotype of the ctt1/nth1 mutant.  The ctt1/apn1 and ctt1/uve1 mutants also had high mutation rates, even though each single mutant showed no mutator phenotype.  Our results provide evidences that BER enzymes as well as calatase and antioxidant contribute in vivo to avoidance of ROS-induced mutagenesis and cell death.


発表雑誌等の名称

Genes and Environment

出版者

日本環境変異原学会

30

3

開始ページ

86

終了ページ

91

発行又は発表の年月

2008/09

査読の有無

有り

招待の有無

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID