論文

基本情報

氏名 池田 正五
氏名(カナ) イケダ シヨウゴ
氏名(英語) Ikeda Shiyogo
所属 生命科学部 生物科学科
職名 教授
researchmap研究者コード 1000113908
researchmap機関 岡山理科大学

題名

Enzyme activity of O-sialoglycoprotein endopeptidase (OSGEP) of Saccharomyces cerevisiae Kae1p is essential for growth, but the bacterial and mammalian OSGEP homologs can not complement the yeast KAE1 null mutation

単著・共著の別

著者

Ikeda, S., Uda, H., and Seki, Y.

概要

O-sialoglycoprotein endopeptidase (OSGEP) is conserved throughout almost all species like eubacteria, archaebacteria, and eukaryotes. A homolog (KAE1) of Saccharomyces cerevisiae is the essential gene for growth of the cells. Here, we designed a complementation system of KAE1- lethality using an S. cerevisiae KAE1+/- heterozygous strain. After introduction of an expression plasmid pAUR123 into the heterozygote, KAE1+ and KAE1- alleles were segregated by random spore analysis. The expression plasmid has a centromere sequence of S. cerevisiae, so is divided to each haploid cell at the same ratio. The expression of Kae1p from the plasmid could complement the lethality of KAE1 null mutation. Alanine substitution of histidine residues in a common motif of glycoprotease M22 family abolished the complementation ability of Kae1p. Therefore, the glycoprotease activity of Kae1p is essential for growth of the yeast cells. Escherichia coli and mouse OSGEP homologs also have well-conserved M22 family motifs, but did not complement the KAE1- lethality in the yeast strain. This suggests that not only the enzymatic activity of OSGEP but also the specific interaction to other proteins is critical for function of yeast OSGEP.


発表雑誌等の名称

The Bulletin of Okayama University of Science

出版者

44

A

開始ページ

27

終了ページ

31

発行又は発表の年月

2009/03

査読の有無

無し

招待の有無

無し

記述言語

英語

掲載種別

研究論文(大学,研究機関等紀要)

ISSN

ID:DOI

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID