|
|
|
| 基本情報 |
|
| 氏名 |
池田 正五 |
| 氏名（カナ） |
ｲｹﾀﾞ ｼﾖｳｺﾞ |
| 氏名（英語） |
Ikeda Shiyogo |
| 所属 |
生命科学部 生物科学科 |
| 職名 |
教授 |
| researchmap研究者コード |
1000113908 |
| researchmap機関 |
岡山理科大学 |
Effect of a selectable marker ura4+ gene on survival of stationary-phase cells of the fission yeast cultured in a synthetic defined medium.
Takanori Senoo, Naoya Katayama, Atori Nakamura, Shinji Kawano, and Shogo Ikeda
The chronological lifespan (CLS) in yeast is defined as the survival time of non-dividing cells such as cells in the stationary phase during growth. Deletion mutants of the fission yeast (Schizosaccharomyces pombe) have been constructed by replacement of target gene with a selectable marker such as the ura4+gene. During CLS assays of various mutants, we noticed that the ura4+ marker might have affected the survival of stationary-phase cells when mutant cells were cultured in a synthetic defined (SD) medium. In this study, we compared the CLS of ura4+ strains with that of several ura4– mutants and show that the ura4+ gene may reduce the survival of stationary-phase cells cultured in SD medium. The shortened CLS phenotype of the ura4+ strain was not observed when cells were cultured in a rich medium YES. Although the reason for this phenomenon is unclear, caution must be taken when the CLS of deletion mutants carrying the ura4+ marker is measured in SD medium.
The Bulletin of Okayama University of Science
|
