N-glucosylation of indole-3-acetyl amino acids modulates auxin metabolism and growth traits in Oryza sativa

Zenji A, Hata K, Segami S, Makita N, Kojima M, Yoshino-Kida M, Nagai K, Ashikari M, Matsubara K, Yano M, Fukuoka S, Hayashi KI, Sato Y, Hobo T, Inukai Y, Sakakibara H.

Dynamic regulation of auxin (indole-3-acetic acid; IAA) levels is crucial for proper plant growth and development and is finely regulated through biosynthesis, transport, metabolic inactivation, and signal transduction. While O-glucosylation of IAA is well established, the physiological significance of N-glucosylation pathways acting on IAA-amino acid conjugates remains largely unexplored. Here, we identify a UDP-glucosyltransferase in rice (Oryza sativa), designated IAAspGT, that catalyzes the N-glucosylation of indole-3-acetyl (IA) amino acid conjugates. Functional analysis revealed that natural allelic variants of IAAspGT differ markedly in enzymatic activity, with the high-activity allele prevalent in indica and the low-activity allele in japonica cultivars. Mutational analysis identified key residues within the glucose-accepting substrate-binding domain that account for this variation. A metabolic perturbation experiment demonstrated that IAAspGT competes with the DAO-mediated oxidation, thereby affecting active auxin levels. Plants harboring the high-activity allele exhibited enhanced root elongation under nutrient-deficient conditions and altered growth allocation between the panicle and other above-ground organs. Haplotype analysis indicated that the high-activity allele is ancestral and widely retained in the wild relatives. These findings establish IA-amino acid N-glucosylation as a previously overlooked metabolic branch that modulates auxin availability and contributes to environmental responses and growth plasticity in rice.

Proc Natl Acad Sci U S A .

123

12

e2527570123

doi: 10.1073/pnas.2527570123.