論文

基本情報

氏名 窪木 厚人
氏名(カナ) クボキ アツヒト
氏名(英語) Kuboki Atsuhito
所属 生命科学部 生物科学科
職名 准教授
researchmap研究者コード 1000311530
researchmap機関 岡山理科大学

題名

Transdermal delivery of paclitaxel-anionic nanoparticles to epidermis layer, pterostilbene, and pterostilbene glycoside, and their application for treatment of skin cancer and wrinkle

単著・共著の別

共著

著者

Hamada, Hiroki ; Uesugi, Daisuke; Ishihara, Kohji; Hosoda, Ryusuke; Shimoda, Kei; Kuboki, Atsuhito; Kiriake, Yuya

概要

Composite nanoparticles, "anionic bicelles", composed of anionic phospholipid of 1,2- dipalmitoyl- sn-glycero-3-phosphorylglycerol (DPPG) and paclitaxel were prepared by mixing them in water and a subsequent heating/cooling/ultrasonicating process. Anionic DPPG-paclitaxel nanoparticles (particle size: 12 nm) could be prepared after ultrasonic fragmentation at low temperature of 4°C. Upon addition of fluorescently labeled paclitaxel nanoparticles stabilized with DPPG to rat skin tissue (in vitro), the nanoparticles "anionic bicelles" (particle size: 12 nm) infiltrated into the epidermis layer penetrating stratum corneum (intercellular space: ca. 100 nm). In addition, during the anti-skin cancer test using mouse model of skin cancer, our study revealed that the numbers of papillomas of the mouse applied with paclitaxel nanoparticles stabilized with DPPG, "anionic bicelles", to mouse skin were decreased, although those of the mouse applied with paclitaxel itself to mouse skin (control) were increased. Thus, this study established that since paclitaxel nanoparticles stabilized with DPPG could permeate stratum corneum and be incorporated into the epidermis layer of mouse, they could also treat skin cancer (in vivo). On the other hand, a mixture of anionic phospholipids, sodium cholate and Technol PG, allowed for formation of anionic nanoparticles, "anionic liposomes", (particle size: 3 nm). This study established that as pterostilbene and pterostilbene glycoside could permeate stratum corneum and be incorporated into the epidermis layer, they could also treat wrinkle.

発表雑誌等の名称

International Journal of Current Microbiology and Applied Sciences

出版者

Excellent Publishers

13

4

開始ページ

1

終了ページ

7

発行又は発表の年月

2024/04

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID