Transdermal delivery of anionic nanoparticles of resveratrol, cannabidiol, paclitaxel and paclitaxel glucoside to epidermis and their application for treatment of skin cancer

Hamada, Hiroki ; Uesugi, Daisuke; Ishihara, Kohji; Hosoda, Ryusuke; Shimoda, Kei; Kuboki, Atsuhito; Kiriake, Yuya

Technol PG-resveratrol or paclitaxel glucoside nanoparticles were successfully prepared by dispersing Technol PG powder with cholic acid-based surfactants such as SC. Composite nanoparticles composed of an anionic phospholipid of 1,2-dipalmitoyl-sn-glycero-3- phosphorylglycerol (DPPG) and resveratrol or paclitaxel were successfully prepared by mixing them in water followed by a subsequent heating and cooling process. Upon addition of small-sized fluorescently labeled resveratrol nanoparticles with DPPG to rat skin tissue, fluorescently labeled resveratrol mols. permeated to the stratum corneum (in vitro). DPPG-paclitaxel nanoparticles exerted high anti-skin cancer activity (in vitro). Technol PG-paclitaxel glucoside nanoparticles also showed high anti-skin cancer activity (in vitro). During the anti-cancer test using mouse model of skin cancer, this study revealed that the numbers of papillomas of the mouse treated with paclitaxel glucoside nanoparticles stabilized with Technol PG were decreased, although those of the mouse treated with paclitaxel glucoside itself were increased. Thus, our study established that since paclitaxel glucoside nanoparticles stabilized with Technol PG could permeate stratum corneum and be incorporated into the epidermis layer of mouse, they could also treat skin cancer (in vivo). This study showed that since DPPG-resveratrol nanoparticles could permeate stratum corneum and be incorporated into the epidermis layer of mouse, they could also treat atopic dermatitis.

International Journal of Current Microbiology and Applied Sciences

Excellent Publishers

13

3

125

132