MISC

基本情報

氏名 古賀 雄一
氏名(カナ) コガ ユウイチ
氏名(英語) Koga Yuuichi
所属 工学部 応用化学科
職名 教授
researchmap研究者コード 5000076449
researchmap機関 岡山理科大学

題名

Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase

単著・共著の別

 

著者

Clement Angkawidjaja
Dong-Ju You
Hiroyoshi Matsumura
Yuichi Koga
Kazufumi Takano
Shigenori Kanaya

概要

A family I.3 lipase from Pseudomonas sp. MIS38 was secreted from Escherichia coli cells to the external medium, purified and crystallized and preliminary crystallographic studies were performed. The crystal was grown at 277 K by the hanging-drop vapour-diffusion method. Native X-ray diffraction data were collected to 1.7 Å resolution using synchrotron radiation at station BL38B1, SPring-8. The crystal belongs to space group P21, with unit-cell parameters a = 48.79, b = 84.06, c = 87.04 Å. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient V M was calculated to be 2.73 Å3 Da-1 and the solvent content was 55%. © International Union of Crystallography 2007.

発表雑誌等の名称

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

出版者

 

63

3

開始ページ

187

終了ページ

189

発行又は発表の年月

2007-02-13

査読の有無

無し

依頼の有無

無し

記述言語

英語

掲載種別

 

ISSN

 

ID:DOI

10.1107/S1744309107004575

ID:NAID(CiNiiのID)

 

ID:PMID

 

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID