Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube.

Masafumi Kashii
Ryo Fujisawa
Hiroaki Adachi
Yusuke Mori
Takatomo Sasaki
Yuichi Koga
Kazufumi Takano
Shigenori Kanaya
Hiroyoshi Matsumura
Tsuyoshi Inoue
Satoshi Murakami
Kazuomi Sugamoto
Hideki Yoshikawa

We developed a novel technique for detaching protein crystals from glass capillary tube using the counter diffusion crystallization technique by femtosecond laser irradiation. X-ray diffraction analysis demonstrated that femtosecond laser irradiation has little effect on crystallinity. This technique will contribute to progress in structural genomics as a powerful tool.

Journal of bioscience and bioengineering

SOC BIOSCIENCE BIOENGINEERING JAPAN

102

4

10.1263/jbb.102.372

https://doi.org/10.1263/jbb.102.372
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000242754800018&DestApp=WOS_CPL
https://www.ncbi.nlm.nih.gov/pubmed/17116588