論文

基本情報

氏名 竹谷 浩介
氏名(カナ) タケヤ コウスケ
氏名(英語) Takeya Kosuke
所属 獣医学部 獣医学科
職名 講師
researchmap研究者コード 5000068922
researchmap機関 岡山理科大学

題名

Highly sensitive myosin phosphorylation analysis in the renal afferent arteriole.

単著・共著の別

単著

著者

Kosuke Takeya

概要

The regulation of smooth muscle contraction and relaxation involves phosphorylation and dephosphorylation of regulatory proteins, particularly myosin. To elucidate the regulatory mechanisms, analyzing the phosphorylation signal transduction is crucial. Although a pharmacological approach with selective inhibitors is sensitive and a useful technique, it leads to speculation regarding a signaling pathway but does not provide direct evidence of changes at a molecular level. We developed a highly sensitive biochemical technique to analyze phosphorylation by adapting Phos-tag SDS-PAGE. With this technique, we successfully analyzed myosin light chain (LC20) phosphorylation in tiny renal afferent arterioles. In the rat afferent arterioles, endothelin-1 (ET-1) induced diphosphorylation of LC20 at Ser19 and Thr18 as well as monophosphorylation at Ser19 via ET2B receptor activation. Considering that LC20 diphosphorylation can decrease the rate of dephosphorylation and thus relaxation, we concluded that LC20 diphosphorylation contributes, at least in part, to the prolonged contraction induced by ET-1 in the renal afferent arteriole.    

発表雑誌等の名称

Journal of Smooth Muscle Research

出版者

52

開始ページ

45

終了ページ

55

発行又は発表の年月

2016/07

査読の有無

有り

招待の有無

有り

記述言語

英語

掲載種別

研究論文(その他学術会議資料等)

ISSN

ID:DOI

10.1540/jsmr.52.45

ID:NAID(CiNiiのID)

ID:PMID

27375035

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID