論文

基本情報

氏名 竹谷 浩介
氏名(カナ) タケヤ コウスケ
氏名(英語) Takeya Kosuke
所属 獣医学部 獣医学科
職名 講師
researchmap研究者コード 5000068922
researchmap機関 岡山理科大学

題名

Phos-tag SDS電気泳動を用いた平滑筋収縮調節タンパク質のリン酸化シグナル解析

単著・共著の別

単著

著者

竹谷 浩介

概要

Activation of myosin by phosphorylation has been implicated in regulation of smooth muscle contractions. The ability to analyze myosin phosphorylation is crucial for better understanding of the regulatory mechanisms of smooth muscle contraction. In myosin phosphorylation analysis, it is important to quantify the ratio between phosphorylated and unphosphorylated myosin because those can have opposite effects on contractions. For this reason, urea/glycerol-PAGE and 2-D electrophoresis in combination with Coomassie blue staining or Western blotting are commonly used to separate and quantify myosin based on its phosphorylated state. However, those conventional techniques are not sensitive enough to analyze minute smooth muscles, such as terminal resistant arterioles, partly because of a poor separation in electrophoresis with a small sample. Recently we overcame this problem by introducing a phos-tag technique, and achieved picogram sensitivity. In this article, the sensitive phosphorylation quantification method and application of phos-tag electrophoresis to smooth muscle physiology research are reviewed.

発表雑誌等の名称

生物物理化学

出版者

56

開始ページ

s15

終了ページ

s19

発行又は発表の年月

2012/01

査読の有無

有り

招待の有無

有り

記述言語

日本語

掲載種別

研究論文(その他学術会議資料等)

ISSN

ID:DOI

10.2198/sbk.56.s15

ID:NAID(CiNiiのID)

ID:PMID

URL

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID