論文

基本情報

氏名 下川 孝子
氏名(カナ) シモカワ タカコ
氏名(英語) Shimokawa Takako
所属 獣医学部 獣医学科
職名 准教授
researchmap研究者コード 7000022171
researchmap機関 岡山理科大学

題名

Intratumoral heterogeneity of c-KIT mutations in a feline splenic mast cell tumor and their functional effects on cell proliferation.

単著・共著の別

 

著者

Yuki Hasegawa
Kazuha Shosu
Kanako Tsuji
Yumiko Shimoyama
Takako Shimokawa Miyama
Kenji Baba
Masaru Okuda
Kazuhito Itamoto
Masaya Igase
Takuya Mizuno

概要

A cat was presented with mast cell tumors (MCTs) of the skin and spleen. During the initial diagnosis, the exon 8 mutation of c-KIT was detected in the masses from skin and spleen by a commercial laboratory test. Consequently, treatment with toceranib was started. After complete remission, because of recurrence on day 117, the spleen and skin tumors were removed, but the cat eventually died on day 191. The analysis of ten cDNA clones of the c-KIT gene cloned from the surgically removed spleen revealed that seven different cDNA patterns were included, indicating the heterogeneity of this gene in the splenic MCT. The seven cDNA nucleotide patterns can be classified into four protein sequence patterns. In addition to the previously known mutations in exon 8, we identified novel mutations in exons 9, 10, and 18; four amino acids deletion in exon 9, and a point mutation in exons 10 and 18. Mouse IL-3-dependent cell line, Ba/F3, was transduced with these mutant clones, and c-KIT phosphorylation and proliferation assays were performed. We found that certain mutations affected the c-KIT phosphorylation status and cell proliferation. This suggests that heterogeneity among the population of tumor cells exists in MCTs, and that the dominant clones of this heterogeneity may contribute to the subsequent tumor cell growth.

発表雑誌等の名称

Scientific reports

出版者

 

12

1

開始ページ

15791

終了ページ

15791

発行又は発表の年月

2022-09-22

査読の有無

有り

招待の有無

 

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

 

ID:DOI

10.1038/s41598-022-19089-5

ID:NAID(CiNiiのID)

 

ID:PMID

 

JGlobalID

 

arXiv ID

 

ORCIDのPut Code

 

DBLP ID