論文

基本情報

氏名 清水 夕貴
氏名(カナ) シミズ ユキ
氏名(英語) Shimizu Yuki
所属 獣医学部 獣医保健看護学科
職名 助教
researchmap研究者コード R000000424
researchmap機関 岡山理科大学

題名

Establishment and characterization of a canine sebaceous epithelial cell line derived from an eyelid mass

単著・共著の別

著者

Akira MATSUDA, Ikki MITSUI, Yuki SHIMIZU, Teppei KANDA, Akihiro OHNISHI, Masahiro MIYABE, Yoshiki ITOH

概要

Little is known about the pathological roles of sebaceous glands in canine skin diseases, as most examinations have been conducted with cultured human sebaceous epithelial cell lines. To our knowledge, there is no available canine sebaceous epithelial cell line. The purpose of this study was to establish a canine sebaceous epithelial cell line and characterize it. An eyelid mass in a dog was surgically resected for treatment, and it was histologically diagnosed as sebaceous epithelioma. Collected tissue was conducted for culture, and the growing epithelial-like cells were passaged. The cells showed continuous proliferation for over 6 months. After 40 passages, the cells were named CMG-1. Lipid droplets in the cytoplasm of CMG-1 cells were confirmed by Oil Red O staining. As reported in studies with human sebaceous epithelial cell lines, lipogenesis in CMG-1 cells was promoted by linoleic acid, whereas transforming growth factor-β (TGF-β) suppressed it. Additionally, real-time PCR revealed that the expression levels of chemokines and cytokines, including CC chemokine ligand (CCL)-2, CCL-20, CXCL-10, Tumor necrosis factor-α (TNF-α), Interleukin (IL)-1α, IL-1β, and IL-8, were significantly increased in CMG-1 cells following treatment with lipopolysaccharide. In conclusion, we successfully established a new canine sebaceous epithelial cell line. Our data indicated that lipogenesis and inflammatory responses were quantitatively evaluable in this cell line. CMG-1 cells could be useful for the pathological analysis of sebaceous gland diseases in dogs.

発表雑誌等の名称

Journal of Veterinary Medical Science

出版者

Japanese Society of Veterinary Science

82

11

開始ページ

1577

終了ページ

1584

発行又は発表の年月

2020/09

査読の有無

有り

招待の有無

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

10.1292/jvms.20-0179

ID:NAID(CiNiiのID)

ID:PMID

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID