Molecular identification systems as a point - of- care testing (POCT) have been made rapidly in the field of infectious disease of human, allowing for the rapid diagnosis of multiple pathogens. POCT is also becoming popular in the diagnosis of livestock pathogens. For example, immunochromatography kits used for diagnosing highly pathogenic avian influenza and real-time PCR used for diagnosing bovine respiratory disease. Generally, immunochromatography is easy for anyone to use and can be diagnosed anywhere, but its sensitivity and specificity are low. On the other hand, real-time PCR has high sensitivity and specificity, but it requires large and expensive equipment, specialized skills due to the complex procedures, limiting its use to specific facilities. A portable real-time PCR device, PicoGene® PCR1100, is compact, easy to operate and has high sensitivity. In addition, the examination times take only about 20 minutes, allowing for the rapid diagnosis of livestock diseases. This review explains the validation of the implementation using PicoGene® PCR1100 for POCT of bovine viral diarrhea virus and Mycoplasma bovis, which are important pathogens causing bovine respiratory infections.
