論文

基本情報

氏名 小林 宏祐
氏名(カナ) コバヤシ コウスケ
氏名(英語) Kobayashi Kosuke
所属 獣医学部 獣医学科
職名 講師
researchmap研究者コード R000002187
researchmap機関 岡山理科大学

単著・共著の別

著者

Michihito Tagawa ,  Hotaka Hiroi ,  Yuzuki Nakano ,  Riyo Morishita ,  Kosuke Kobayashi ,  Osamu Sakai

概要

Only a limited number of tumour biomarkers are currently available in veterinary medicine, particularly in cats. Cell-free DNA (cfDNA) is an extracellular DNA fragment released upon cell death and is considered a minimally invasive biomarker for the diagnosis and monitoring of various human malignancies. This study aimed to clarify the utility of circulating cfDNA as a liquid biopsy for various feline tumours. Plasma samples were collected from 44 cats with various tumours, 24 cats with other diseases and 10 healthy controls. A follow-up study was conducted in three tumour-bearing patients. All cfDNA concentrations were quantified via real-time polymerase chain reaction (PCR), which provided short and long fragments of a newly identified feline LINE-1 gene. We found that cfDNA levels were significantly higher in cats with various tumours than in those with other diseases or healthy controls. The cfDNA concentration was not correlated with serum amyloid A (SAA) levels. Cats with tumours exhibited elevated cfDNA levels that predicted tumour-bearing with a sensitivity and specificity of 50.5% and 91.2%, respectively (AUC 0.736; p < 0.001). In lymphoma cases, cats with high cfDNA levels had significantly shorter survival times than those with low cfDNA levels (median: 33 days vs. 178 days; p = 0.003). In addition, the cfDNA levels of the three patients correlated with clinical status during follow-up. Collectively, these findings indicate the potential of cfDNA as a useful biomarker for the diagnosis, therapeutic monitoring and prognostic assessment of tumours in cats.

発表雑誌等の名称

Veterinary Comparative Oncology

出版者

22

4

開始ページ

592

終了ページ

601

発行又は発表の年月

2024/12

査読の有無

有り

招待の有無

無し

記述言語

英語

掲載種別

研究論文(学術雑誌)

ISSN

ID:DOI

10.1111/vco.13013

ID:NAID(CiNiiのID)

ID:PMID

JGlobalID

arXiv ID

ORCIDのPut Code

DBLP ID