According to this research, we could establish a simple and useful PCR device. Until then, the cheapest thermal cycler is around 300,000 Japanese yen, but this new developed PCR device is only 100,000 (in fact 80,000 yen). This is the most valuable accomplishment in our study. Furthermore, this newly established device could detect unique DNA bar cording band patterns of invasive ant species derived from Central America. This is much easier than that of typical DNA bar coding using DNA sequencing process. We implemented to the Sapporo Ohtani high school, Hokkaido Science Educational Center, and Chiba Oihama high school with its teachers and it confirmed that this device and experimental procedure are fully effective against environmental education in high school curriculum.
