1. MUC1 derived multi HLA binding peptides, which restricted to HLA-A*24:02, A*02:01, and A*02:06, were identified.
2. Cathepsin B and Calreticulin are highly expressed in pancreatic cancer stem like cells, and might be therapeutic targets against pancreatic cancer stem cells. One and eight Cathepsin B and Calreticulin derived peptides, which restricted to HLA-A*24:02, A*02:01, A*02:06, were identified, respectively.
3. The neoantigens derived from pancreatic cancer were moving before and after chemotherapy. Hence, therapeutic neoantigens should be seeking based on the tumor mutations after chemotherapy. Similarly, tumor mutation state might be changed from primary regions to metastatic regions. Hence, therapeutic neoantigens should be seeking based on the tumor mutations of metastatic lesions.
