The genes related to glycerol metabolism were searched from genomic information of Thermococcus kodakarensis KOD1. From the results, the number of the genes is thought to be smaller than that of bacteria. Following analysis about those genes, e. g. enzymatic characterization, gene disruption and operon structure analysis, indicate that the glycerol metabolic pathway of T. kodakarensis is very simple to compare with that of bacteria or eukarya and have a unique regulation mechanism for the glycerol metabolism.