Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation.

Clement Angkawidjaja
Dong-ju You
Hiroyoshi Matsumura
Katsumasa Kuwahara
Yuichi Koga
Kazufumi Takano
Shigenori Kanaya

The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.

FEBS letters

ELSEVIER SCIENCE BV

581

26

5060

4

10.1016/j.febslet.2007.09.048

https://doi.org/10.1016/j.febslet.2007.09.048
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000250802700009&DestApp=WOS_CPL
https://www.ncbi.nlm.nih.gov/pubmed/17923123