Crystallization and preliminary crystallographic analysis of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7.

Dong Ju You
Hyongi Chon
Yuichi Koga
Kazufumi Takano
Shigenori Kanaya

Crystallization and preliminary crystallographic studies of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7 were performed. A crystal was grown at 277 K by the sitting-drop vapour-diffusion method. Native X-ray diffraction data were collected to 1.5 angstroms resolution using synchrotron radiation from station BL41XU at SPring-8. The crystal belongs to space group P4(3), with unit-cell parameters a = b = 39.21, c = 91.15 angstroms. Assuming the presence of one molecule in the asymmetric unit, the Matthews coefficient V(M) was calculated to be 2.1 angstroms3 Da(-1) and the solvent content was 40.5%. The structure of a selenomethionine Sto-RNase HI mutant obtained using a MAD data set is currently being analysed.

Acta crystallographica. Section F, Structural biology and crystallization communications

62

Pt 8

781

4

10.1107/S1744309106024420

https://doi.org/10.1107/S1744309106024420
https://www.ncbi.nlm.nih.gov/pubmed/16880556
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242919