Academic Thesis

Basic information

Name Koga Yuuichi
Belonging department
Occupation name
researchmap researcher code 5000076449
researchmap agency Okayama University of Science

Title

Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production.

Bibliography Type

 

Author

Kohei Kaneyoshi
Keiji Uchiyama
Masayoshi Onitsuka
Noriko Yamano
Yuichi Koga
Takeshi Omasa

Summary

© 2018 The Society for Biotechnology, Japan The production of biopharmaceutical immunoglobulin G (IgG) using cultured mammalian cells, especially Chinese hamster ovary (CHO) cells is well established and has been markedly improved through the modification of cells and cell culture engineering technologies. The establishment of high-production cell lines remains a challenge. The intracellular secretion of IgG has been investigated to identify and solve the rate-limiting steps in antibody production. However, strategies that regulate the expression of proteins that are related to antibody secretory pathway have not consistently improved their production. In this study, key features and limitations of the antibody secretion process in recombinant CHO cells were analyzed to develop more efficient approaches for establishing high-production cells. By chase assay with protein translation inhibitors, IgG secretion reached a plateau when at least 20% of IgG remained in the cells. The secretion kinetics and retention ratio of IgG varied between IgG subclasses (two types of IgG1 and an IgG3 subclass). Immunofluorescent microscopy and size exclusion chromatography showed that the remaining intracellular IgG localized mainly within the endoplasmic reticulum (ER) and less with the cis-Golgi network, despite the formation of fully assembled IgG. These results show that remaining intracellular IgG is a target for enhancing antibody secretion, even in high-production CHO cells.

Magazine(name)

Journal of Bioscience and Bioengineering

Publisher

 

Volume

127

Number Of Pages

1

StartingPage

107

EndingPage

113

Date of Issue

2019-01

Referee

Exist

Invited

Not exist

Language

English

Thesis Type

Research papers (academic journals)

ISSN

 

DOI

10.1016/j.jbiosc.2018.06.018

NAID

 

PMID

 

J-GLOBAL ID

 

arXiv ID

 

ORCID Put Code

 

DBLP ID